- From 1995-2000, Julianna was nominated for an Emmy Award in the category of Outstanding Lead Actress in a Drama Series for her portrayal of nurse Carol Hathaway on ER . She won the award in 1995.
- Julianna has been nominated for 8 Screen Actors Guild (SAG) Awards for her work on ER. In 1996, 1998 and 1999 she was nominated for Outstanding Performance by a Female Actor in a Drama Series. She won in both 1998 and 1999. From 1997-2001 she, along with the rest of the main cast, was nominated for Outstanding Performance by an Ensemble in a Drama Series. ER won the award in 1997, 1998 and 1999.
- In May 2007, Julianna was chosen as one of People Magazine's annual 100 Most Beautiful People in the World, Beauty at Every Age section, for age 40.
- While starring on ER, Julianna appeared in four of NBC's "The More You Know" public service announcements. Her topics were designated drivers, substance abuse, domestic violence, and sexually transmitted diseases.
- According to Reuters, in 1999 Julianna was offered a $27 million contract to remain on ER for two more seasons, but turned it down to pursue roles in features and theater.
- Julianna is left handed.
- Julianna dated actor Ron Eldard from 1991 until 2003. He had a recurring role on ER in 1995–95, and his character, Paramedic Ray "Shep" Shepard, dated Julianna's character, Nurse Carol Hathaway.
- Julianna is 5 feet 7 inches tall.
- Julianna auditioned for the role of Alison Parker on Melrose Place, as did her former ER co-star Sherry Stringfield.
- Julianna has two older sisters: Alexandra and Rachel. Alexandra is also an actress, and portrayed a flight attendant in Julianna's final ER episode, "Such Sweet Sorrow." Alexandra has also appeared in episodes of Friends, as well as in an episode of The X-Files and an episode of Veronica’s Closet.
- Julianna has received six Golden Globe Award nominations: four for her performances on ER (in 1996, 1998, 1999, and 2000), one for her performance on the television mini-series The Mists of Avalon (in 2002), and one for her performance on the television mini-series The Grid (in 2005).
- In 1998, Julianna was voted one of People magazine's "50 Most Beautiful People."
- Julianna's character on ER, nurse Carol Hathaway, was originally supposed to be in the pilot episode of the series only, where she was scripted to commit suicide. However, test audiences responded so well to her that the producers asked Julianna to stay on the show, and the script was re-written so that her character's "suicide" became a "suicide attempt." Julianna remained a regular cast member for six seasons.
- According to Snopes.com, an urban legends reference site, Julianna's father, Paul Margulies, was an advertising executive who helped create the "Plop-plop, fizz-fizz, oh what a relief it is" campaign for Alka-Seltzer.
- Julianna Margulies: (about September 11, 2001) I needed to do whatever I could. And I did go down there and help. I cooked for the guys. I visited sick people. I felt, I'm from New York and it's my community and it's what I know and I wouldn't have wanted to be anywhere else. (FrontPageMagazine.com; July 20, 2004)
- Julianna Margulies: I've never been one to do anything for the money. If a movie isn't something I want, I will turn it down. I follow my heart, and it always leads me to who I want to be. (CityBeat; Jan. 27-Feb. 2, 2000)
- Julianna Margulies: I don't want to crash and burn before I've gotten to where I want to go. I want to enjoy the ride. (Entertainment Weekly; June 7, 1996)
